Poster: Methods for mRNA poly(A) Sizing Using RNase Digestion & Capillary Gel Electrophoresis, & Nanopore-based Sequencing
Poster Abstract: The poly(A) tail region of an mRNA functions to stabilize the mRNA and enable translation of the protein. Due to its biological importance of an mRNA’s poly(A) tail, the length of the poly(A) tail is typically evaluated as a Critical Quality Attribute for manufactured mRNA medicines. The method described here was developed to…
Poster: Evaluation of Doggybone™ DNA as Template for In Vitro Transcription
Poster Abstract: Linear DNA is used as the template to in vitro transcribe (IVT) synthetic mRNA. Common sources for templates are pDNA linearized with a restriction enzyme and PCR amplicons. Enzymatically produced Doggybone™ DNA (dbDNA), is a novel IVT template that may improve the stability of DNA-templated poly(A) regions. Further, the highly scalable, cell-free process…
Poster: mRNA Capping Technologies – Effects on Quality Attributes, Biological Effects, and Innate Immune Stimulation
Poster Abstract: Synthetic mRNA is more potent, stable, and immuno-silent when the 5’ end contains a Cap1 structure (N7-methyl guanosine connected to the 5′ nucleotide through a 5′ to 5′ triphosphate linkage; N1 nucleotide methylated at the 2’ position). Cap1 can be added to the synthetic mRNA enzymatically using a guanylyltransferase that removes the 5’…
Poster: Novel Plasmid DNA-encoded poly(A) Tails For mRNA Synthesis
Poster Abstract: Poly-adenosine (poly(A)) tails are essential for the translation and stability of mRNA. Most mRNA synthesis processes rely on in vitro transcription(IVT) of mRNA from a linearized plasmid DNA (pDNA) template which includes a region of uninterrupted A-T base pairs that encode the poly(A) tail. The pDNA is maintained, scaled-up, and harvested from a…
Advantages of Cas9 mRNA for Gene Editing: Precision, Control & Cost Effective
CRISPR-Cas9 RNA-DNA complex. Structure of Cas9 endonuclease (yellow-orange) complexed with a guide RNA (blue), a target DNA (pink) and a non-target DNA (green). The revolutionary CRISPR-Cas gene editing technology has unlocked unprecedented possibilities in genome engineering, gene therapy, and cell therapy. The choice between using Cas9 mRNA or Cas9 protein becomes crucial. One has to…
Vernal Announces Successful Multigram mRNA Scale-up at the New GMP Facility
Vernal has successfully demonstrated its ability to manufacture high quality mRNA using CGMP-compliant platform. We now offer the first integrated platform for CGMP production of MCB, pDNA, mRNA, and LNP at our GMP facility. Contact us today to reserve your GMP manufacturing slot.