The mRNA Monthly—June 2024 Newsletter
mRNA Capping TechnologiesExplore this study, which compares two important mRNA capping technologies by evaluating the mRNA integrity, percent Cap1 content, dsRNA content, in vivo expression and innate immune activation in mice. Download the Poster 7 Key Benefits of a US-Based CDMOIn the fast-paced world of biopharmaceuticals, choosing the right contract development and manufacturing organization (CDMO)…
The Benefits of Partnering with a US-Based CDMO: Quality, Efficiency, and Peace of Mind
In the fast-paced world of biopharmaceuticals, choosing the right contract development and manufacturing organization (CDMO) is crucial to the success of your drug development program. At Vernal Biosciences, we pride ourselves on being a US-based CDMO that produces all products domestically. Here are key benefits of partnering with a CDMO like Vernal Biosciences: 1. Legal and Ethical StandardsCountries…
The mRNA Monthly—May 2024 Newsletter
From the FounderWelcome to the NEW monthly mRNA newsletter! In this monthly update, we will share the latest breakthroughs, insights, and innovations in mRNA and mRNA-LNP technology. By reading these updates, you will gain a competitive edge, stay ahead of industry trends, and unlock new opportunities in the rapidly evolving field of mRNA therapeutics. Together,…
Poster: Methods for mRNA poly(A) Sizing Using RNase Digestion & Capillary Gel Electrophoresis, & Nanopore-based Sequencing
Poster Abstract: The poly(A) tail region of an mRNA functions to stabilize the mRNA and enable translation of the protein. Due to its biological importance of an mRNA’s poly(A) tail, the length of the poly(A) tail is typically evaluated as a Critical Quality Attribute for manufactured mRNA medicines. The method described here was developed to…
Poster: Evaluation of Doggybone™ DNA as Template for In Vitro Transcription
Poster Abstract: Linear DNA is used as the template to in vitro transcribe (IVT) synthetic mRNA. Common sources for templates are pDNA linearized with a restriction enzyme and PCR amplicons. Enzymatically produced Doggybone™ DNA (dbDNA), is a novel IVT template that may improve the stability of DNA-templated poly(A) regions. Further, the highly scalable, cell-free process…
Poster: mRNA Capping Technologies – Effects on Quality Attributes, Biological Effects, and Innate Immune Stimulation
Poster Abstract: Synthetic mRNA is more potent, stable, and immuno-silent when the 5’ end contains a Cap1 structure (N7-methyl guanosine connected to the 5′ nucleotide through a 5′ to 5′ triphosphate linkage; N1 nucleotide methylated at the 2’ position). Cap1 can be added to the synthetic mRNA enzymatically using a guanylyltransferase that removes the 5’…