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Poster: Evaluation of Doggybone™ DNA as Template for In Vitro Transcription

Poster Abstract: Linear DNA is used as the template to in vitro transcribe (IVT) synthetic mRNA. Common sources for templates are pDNA linearized with a restriction enzyme and PCR amplicons. Enzymatically produced Doggybone™ DNA (dbDNA), is a novel IVT template that may improve the stability of DNA-templated poly(A) regions. Further, the highly scalable, cell-free process…

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Poster: mRNA Capping Technologies – Effects on Quality Attributes, Biological Effects, and Innate Immune Stimulation

Poster Abstract: Synthetic mRNA is more potent, stable, and immuno-silent when the 5’ end contains a Cap1 structure (N7-methyl guanosine connected to the 5′ nucleotide through a 5′ to 5′ triphosphate linkage; N1 nucleotide methylated at the 2’ position). Cap1 can be added to the synthetic mRNA enzymatically using a guanylyltransferase that removes the 5’…

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Poster: Novel Plasmid DNA-encoded poly(A) Tails ​For mRNA Synthesis

Poster Abstract: Poly-adenosine (poly(A)) tails are essential for the translation and stability of mRNA. Most mRNA synthesis processes rely on in vitro transcription(IVT) of mRNA from a linearized plasmid DNA (pDNA) template which includes a region of uninterrupted A-T base pairs that encode the poly(A) tail. The pDNA is maintained, scaled-up, and harvested from a…

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Advantages of Cas9 mRNA for Gene Editing: Precision, Control & Cost Effective 

CRISPR-Cas9 RNA-DNA complex. Structure of Cas9 endonuclease (yellow-orange) complexed with a guide RNA (blue), a target DNA (pink) and a non-target DNA (green).  The revolutionary CRISPR-Cas gene editing technology has unlocked unprecedented possibilities in genome engineering, gene therapy, and cell therapy. The choice between using Cas9 mRNA or Cas9 protein becomes crucial. One has to…

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