eGFP mRNA Quantity

$366.00$7,169.00

Vernal now offers catalog eGFP mRNA that has been optimized to produce high expression level of the enhanced green fluorescent protein. Originally isolated from the jellyfish, Aequorea victoria, eGFP is a powerful reporter molecule broadly utilized for monitoring gene expression and protein localization.  eGFP displays bright green fluorescence when exposed to light in the blue to ultraviolet range, with an emissions peak at 509 nm. Vernal’s eGFP mRNA is ready to use for a wide variety of functional studies including biodistribution (in vivo imaging), gene regulation, cell viability, and translation efficiency.

Vernal now offers catalog eGFP mRNA that has been optimized to produce high expression level of the enhanced green fluorescent protein. Originally isolated from the jellyfish, Aequorea victoria, eGFP is a powerful reporter molecule broadly utilized for monitoring gene expression and protein localization.  eGFP displays bright green fluorescence when exposed to light in the blue to ultraviolet range, with an emissions peak at 509 nm. Vernal’s eGFP mRNA is ready to use for a wide variety of functional studies including biodistribution (in vivo imaging), gene regulation, cell viability, and translation efficiency.

Vernal’s mRNAs are highly pure mRNAs that incorporate modified nucleoside (N1-methyl-pseudouridine), Cap-1 structure, optimized codons and poly(A) tail to improve translation efficiency and reduce host cell immune response. Vernal’s mRNAs function as mature, fully processed mRNAs, and are ready for use in cells and animals.

Catalog #VRN-EGFP
Gene nameeGFP
Origin/SpeciesAequorea victoria, Jellyfish
AppearanceClear and colorless
Encoded ProteinEnhanced green fluorescent protein
FunctionReporter Gene
Codon OptimizationYes
PurityFragment analysis by capillary electrophoresis
Length995 bp
Base compositionModified
Concentration260/280 absorbance ratio
BufferWater
Recommended StorageAt -70°C or below
ApplicationBiodistribution, gene regulation, cell viability, translation efficiency
Other NamesGFP mRNA, Green fluorescent protein mRNA
General Tips Thaw on ice. Avoid freeze thaws as much as possible, centrifuge and aliquot into use volumes. Take precautions to avoid Rnase, use Rnase free reagents and consumables with appropriate Rnase free technique.
Shipping ConditionsShip on dry ice at -70°C or below
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Appearance
mRNA Identity
mRNA Concentration
mRNA Integrity
dsRNA
Residual DNA template
5’ Capping Efficiency 
pH 
Endotoxin 
Bioburden* 
Residual Protein