Molecular model of the bioluminescent enzyme FLuc complexed with an intermediate protein.

FLuc mRNA

From: $305.00

Vernal now offers catalog firefly luciferase (FLuc) mRNA that has been optimized to produce high expression level of luciferase enzyme. Originally isolated from the firefly, Photinus pyralis, luciferase catalyzes the oxidation of luciferin, (O2 & ATP dependent), leading to chemiluminescence at 550 nm-570 nm. FLuc can be utilized for a wide variety of functional studies including biodistribution (in vivo imaging), gene regulation, cell viability and translation efficiency.

Variation SKU Price
5MG VRN-FLUC-5MG $5,999.00
1MG VRN-FLUC-1MG $1,673.00
500UG VRN-FLUC-500UG $1,068.00
250UG VRN-FLUC-250UG $610.00
100UG VRN-FLUC-100UG $305.00

Vernal now offers catalog firefly luciferase (FLuc) mRNA that has been optimized to produce high expression level of luciferase enzyme. Originally isolated from the firefly, Photinus pyralis, luciferase catalyzes the oxidation of luciferin, (O2 & ATP dependent), leading to chemiluminescence at 550 nm – 570 nm. FLuc can be utilized for a wide variety of functional studies including biodistribution (in vivo imaging), gene regulation, cell viability and translation efficiency.

Vernal’s mRNAs are highly pure mRNAs that incorporate modified nucleoside (N1-methyl-pseudouridine), Cap-1 structure, optimized codons and poly(A) tail to improve translation efficiency and reduce host cell immune response. Vernal’s mRNAs function as mature, fully processed mRNAs, and are ready for use in cells and animals.

Concentration and integrity testing assays are provided as standard.

Catalog #VRN-FLUC
Gene NameFLuc
Origin/SpeciesPhotinus pyralis, firefly
AppearanceClear and colorless
Encoded ProteinLuciferase
FunctionReporter Gene
Codon OptimizationYes
PurityFragment analysis by capillary electrophoresis
Length2009 bp
Base CompositionModified
Concentration260/280 absorbance ratio
BufferWater
Recommended StorageAt -70°C or below
ApplicationBiodistribution, gene regulation, cell viability, translation efficiency
Other NamesFirefly Luciferase mRNA, FLuc mRNA
General Tips Thaw on ice. Avoid freeze thaws as much as possible, centrifuge and aliquot into use volumes. Take precautions to avoid Rnase, use Rnase free reagents and consumables with appropriate Rnase free technique.
Shipping ConditionsShip on dry ice at -70°C or below
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Test
mRNA Concentration
mRNA Integrity
dsRNA
Residual DNA template
5’ Capping Efficiency 
Endotoxin 
Residual Protein

Test For research use only. Not for use in humans. The purchase of Vernal products does not grant or imply any license under any patents or patent pending. Vernal does not grant or imply and license under any patent or patent pending. Vernal does not warrant that the use or sale of products will not infringe the claims of any patents or patents pending. The purchaser has sole responsibility for all and any use of Vernal products.

Biodistribution Study using Vernal’s Firefly Luciferase