Poster Abstract: Linear DNA is used as the template to in vitro transcribe (IVT) synthetic mRNA. Common sources for templates are pDNA linearized with a restriction enzyme and PCR amplicons. Enzymatically produced Doggybone™ DNA (dbDNA), is a novel IVT template…
Poster Abstract: Synthetic mRNA is more potent, stable, and immuno-silent when the 5’ end contains a Cap1 structure (N7-methyl guanosine connected to the 5′ nucleotide through a 5′ to 5′ triphosphate linkage; N1 nucleotide methylated at the 2’ position). Cap1…
Poster Abstract: Poly-adenosine (poly(A)) tails are essential for the translation and stability of mRNA. Most mRNA synthesis processes rely on in vitro transcription(IVT) of mRNA from a linearized plasmid DNA (pDNA) template which includes a region of uninterrupted A-T base…